Want to know more about our comprehensive microbiome assay kits? It’s our goal to get you up and running in no time. Read our FAQ below. If you still have questions, contact Technical Support at email@example.com.
What is Shoreline Biome?
Shoreline Biome is an early-stage company developing leading-edge microbiome technologies and products designed to advance microbiome research. Shoreline Biome provides easy-to-use, cost-effective tools and reagents that support end-to-end microbiome analysis. Shoreline Biome has developed the first comprehensive and easy-to-use solution for all-in-one microbe lysis, nucleic acid extraction, and 16S PCR amplification from complex samples for sequencing on both the Illumina and PacBio sequencing platforms. Since its inception, Shoreline Biome’s goal has been to create products that allow researchers to get to their science faster with more complete and comprehensive data. Researchers at the forefront of microbiome research are using Shoreline Biome kits to generate the highest-quality and most-complete data sets without added time and expense.
Where is Shoreline Biome located?
Shoreline Biome is located in Farmington, Connecticut, at the University of Connecticut Technology Incubator Program (UConn TIP). The UConn TIP is within walking distance of the University of Connecticut Health Center and The Jackson Laboratory for Genomic Medicine.
How do I purchase a Shoreline Biome assay kit?
Shoreline Biome assay kits can be purchased via credit card or purchase order from our secure online store. For quotes and larger orders, please contact Karen Woodward, VP of Business Development, at firstname.lastname@example.org.
What is included in Shoreline Biome assay kits?
Each Shoreline Biome assay kit includes the reagents and barcoded primers needed to easily and quickly prepare amplicons for 16S sequencing. The assay kits for the Illumina sequencing platform contain one 96-well plate for lysis, one tube of DNA purification beads, and one 96-well PCR plate containing 96 barcoded primers targeting either the V1–V3 or V4 region of the 16S rRNA gene. Following PCR amplification, the amplicons containing the Illumina sequencing adaptor are pooled, purified, and are ready for sequencing. The assay kits for the PacBio sequencing platform contain one 96-well plate for lysis, one tube of DNA purification beads, and one 96-well PCR plate containing 96 barcoded primers targeting either the full 16S rRNA gene or the 16S-ITS-23S region. Following PCR amplification, the amplicons are pooled and ready for the PacBio SMRTbell reaction.
Why are Shoreline Biome assay kits better than others on the market?
Shoreline Biome kits provide the most-complete and easy-to-use microbiome sequencing solution on the market. Experienced and novice microbiome researchers alike trust Shoreline Biome assay kits for fast, comprehensive data results. Shoreline Biome kits allow researchers to quickly generate the amplicons required for their short- or long-read sequencing data. The kits provide the highest-quality 16S amplicon preps available, prepared for pooling and sequencing on either the Illumina or PacBio sequencing platform. In head-to-head comparisons, Shoreline Biome’s proprietary technology has identified organisms that other kits could not. It is not necessary to weigh samples prior to starting the process. The kits allow for simple lysis of up to 96 samples with comprehensive representation of Gram-positive Firmicutes and other difficult-to-lyse microbes in under 35 minutes. They are configured in a 96-well plate format that provides a seamless transition to single-step PCR. Unique, dual-matched barcode designs cover known PCR primer variants while limiting PCR bias and maintaining proportional representation. When used with the PacBio platform, Shoreline Biome's intuitive analysis software and specially-curated Athena database allow a straightforward analysis of the sequence data with superior high-resolution readouts.
I use an Illumina sequencing platform. Are Shoreline Biome assay kits right for me?
Shoreline Biome has two kits for purchase that support Illumina 16S sequencing. The Shoreline Complete V1-V3 Kit provides reagents for sequencing the V1–V3 region of 16S. The Shoreline Complete V4 kit provides PCR reagents for sequencing the V4 region of 16S. Both kits include Illumina sequencing adaptors which are added to the amplicons during the PCR step. Additionally, both kits output 96 barcode-labeled amplicons ready for Illumina sequencing.
I use a PacBio or other long-read sequencing platform. Are Shoreline Biome assay kits right for me?
Shoreline Biome has two kits for purchase that support PacBio sequencing. The Shoreline Complete V1-V9 Kit supports full-length 16S. This kit yields SMRTbell-ready amplicons of approximately 1,500 bp. The full-length 16S amplicon generally leads to identification of microbes at the species level. The Shoreline Complete StrainID Kit supports 16S-ITS-23S. This kit yields SMRTbell-ready amplicons of approximately 2,500 bp. The StrainID amplicon allows identification of microbes at the strain level.
Are there any restrictions on the use of Shoreline Biome assay kits?
Shoreline Biome kits are currently available for research use only and are not for redistribution. If you are interested in using Shoreline Biome kits for diagnostic assay development, please contact us at email@example.com.
I have a question about your software. How can I contact technical support?
I ordered a Shoreline Biome assay kit. When will I receive it?
Most orders are shipped within 2–3 working days of order placement. Each customer is provided an estimate of delivery at time of order. Orders placed on Friday will not be shipped until the following Monday.
Do you ship kits internationally?
Currently, we do not ship outside the U.S. If your laboratory or business is located outside of the U.S., please contact our business development team to receive information regarding U.S. service providers who can assist with your project. If you are interested in international distribution, please contact our business development team at firstname.lastname@example.org.
I am interested in collaborating on a research project. Who should I contact?
Please contact us via email at email@example.com.
I would like to purchase kits in bulk. How do I request a quote for a bulk order?
Please contact Karen Woodward, VP of Business Development, for a quote. Her email is firstname.lastname@example.org.