Can I obtain and visualize DADA2 Amplicon Sequence Variants (ASVs) from StrainID and V1V9 data?

Yes, but you must have R and SBanalyzer version 3 installed to be able to infer ASVs from your taxa of interest. Demultiplexed FASTQ files from SBanalyzer v3 can be used in DADA2 ( to infer ASVs from bacteria in samples. For additional instructions please contact

Reference: Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJ, Holmes SP. DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 2016 Jul;13(7):581-3. doi: 10.1038/nmeth.3869. Epub 2016 May 23. PMID: 27214047; PMCID: PMC492737

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