Do your primers cover all bacteria?

To build our primer sets, we performed a search of the SILVA database (hundreds of thousands of complete and partial 16S genes), the Riken database (2,275 complete and 5,664 draft genome sequences including the Human Microbiome Project (HMP) genome sequences data from NCBI), the Athena database which has 18,566 fully assembled genomes from RefSeq database (Dec. 2018).

The databases include organisms from many different sources including human oral, fecal, skin, model organisms, environment, etc. Our design includes all known (sequenced) variants and prospective unknown primer variants based on the rRNA gene structure. We also have a novel primer design that limits the effects of potential primer depletion or competition.

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