What are the recommended controls?

Researchers can choose their own microbiome controls. We have several that we’ve used successfully from Zymo and ATCC.

• Process positive control: ATCC MSA-2002, MSA2006, Zymo D6300
• Process negative control: water at the lysis step
• PCR positive control: MSA 1002, MSA1006, Zymo D6305
• PCR negative control: water at the PCR step

Shoreline Biome recommends including controls in your project to monitor lysis and amplification efficiency, as well as environmental contamination.