What are the recommended controls?

Researchers can choose their own microbiome controls. We have several that we’ve used successfully from Zymo and ATCC.

  • Process positive control: ATCC MSA-2002, MSA2006, Zymo D6300
  • Process negative control: water at the lysis step
  • PCR positive control: MSA 1002, MSA1006, Zymo D6305
  • PCR negative control: water at the PCR step

Shoreline Biome recommends including controls in your project to monitor lysis and amplification efficiency, as well as environmental contamination.

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