Researchers can choose their own microbiome controls. We have several that we’ve used successfully from Zymo and ATCC.
- Process positive control: ATCC MSA-2002, MSA2006, Zymo D6300
- Process negative control: water at the lysis step
- PCR positive control: MSA 1002, MSA1006, Zymo D6305
- PCR negative control: water at the PCR step
Shoreline Biome recommends including controls in your project to monitor lysis and amplification efficiency, as well as environmental contamination.