The Shoreline Complete kits contain a Lyse & Purify sub-kit and an Amplify sub-kit.
The Lyse & Purify Sub-kit contains:
- a 96-well plate or 16 tubes for lysis
- 2 bottles or tubes of DNA purification beads
The Amplify Sub-kit contains:
- a PCR plate seal for amplification
- a 96-well PCR plate containing 96 barcoded primers or 16 tubes containing 16 barcoded primers
For Illumina, these primers target either the V1–V3 or V4 region of the 16S rRNA gene. Following PCR amplification, the amplicons containing the Illumina sequencing adaptor are pooled, purified, and are ready for sequencing.
For PacBio, these primers target either the full 16S rRNA gene or the 16S-ITS-23S region. Following PCR amplification, the amplicons are pooled and ready for the PacBio SMRTbell reaction.