Shoreline Biome kits have been optimized for low-sample inputs. Adding too much sample could cause the assay to fail. The following guidelines are recommended for sample input lysis:
|Sample Type||Input Amount||Maximum|
|Solid Fecal||1-3 mg||10 mg|
|Liquid Fecal||10 µL||10 µL|
|DNA Genotek OMNIgene®•Gut||10 µL||10 µL|
|Tissue||20 mg||30 mg|
|Mouse Fecal||5 mg (1/4 pellet)||10 mg (1/2 pellet)|
Buffer Considerations: The Shoreline Rapid Prep and Shoreline Complete lysis step is pH dependent, therefore it is important to follow recommended guidelines for sample input. If sample is stored in buffer with pH < 6.5, 10 µL is the maximum input volume. Adding more than this volume can result in suboptimal lysis and affect downstream results.