A More Accurate, Cheaper, Faster Microbiome Preparation Method

Download our joint poster with The Jackson Laboratory titled “A More Accurate, Cheaper, Faster Microbiome Preparation Method: Application to Study the Microbiome in Cocaine Addiction” presented at ASM 2019.

Sequence based profiling of microbiomes requires comprehensive microbial lysis. A DNA extraction method that is stringent enough to lyse all cells while not damaging DNA is needed, especially for long read applications. We report results of a non-bead beating, non-enzymatic, novel ‘Rapid’ microbiome DNA extraction procedure suitable for 16S rRNA gene based microbiome profiling applications.