Shoreline Biome’s all-in-one, easy-to-use microbiome assay kits are the solution for researchers at all levels seeking complete, high-quality microbiome assay data sets. Our kits are fast, complete, and uncomplicated systems designed to transform your complex samples from lysis to sequencing to analysis with precision barcoded results.

Researchers at the forefront of microbiome research trust our kits to generate reliable data while reducing sample handling time and eliminating the need to build their own end-to-end solution.

Shoreline Biome assay kits provide significant improvement over many of the standard protocols that are known to miss microbes or provide biased results. All aspects of the systems are designed from the ground up to work together on the industry leading long read PacBio and short read Illumina sequencing platforms.

Each Shoreline Biome assay kit includes the reagents and barcoded primers needed to easily and quickly lyse samples and prepare amplicons for 16S sequencing.

The assay kits for the Illumina sequencing platform contain one 96-well plate for lysis, one tube of DNA purification beads, and one 96-well PCR plate containing 96 barcoded primers targeting either the V1–V3 or V4 region of the 16S rRNA gene. Following PCR amplification, the amplicons containing the Illumina sequencing adaptor are pooled, purified, and are ready for sequencing.

The assay kits for the PacBio sequencing platform contain one 96-well plate for lysis, one tube of DNA purification beads, and one 96-well PCR plate containing 96 barcoded primers targeting either the full 16S rRNA gene or the 16S-ITS-23S region. Following PCR amplification, the amplicons are pooled and ready for the PacBio SMRTbell reaction.  There are additional 16-sample kits for both the full 16S and the 16S-ITS-23S that can be combined with the corresponding 96-well kit, or run separately.

Shoreline Biome, microbiome

THE TECHNOLOGY

Shoreline Biome’s proprietary technology combines a series of innovations; lysis, DNA prep, and PCR to significantly reduce hands-on time and time to result while improving coverage and representation of bacteria in microbiome samples.

While many lysis techniques fail to uniformly open the diverse array of cells in microbiome samples, Shoreline Biome’s lysis process rapidly and uniformly extracts DNA with very little handling. Our kit’s single-step PCR (with accompanying DNA barcodes) allows pooling of over 100 samples per run. And our software, provided with each PacBio kit, presents a GUI optimized for barcode ID, sample sorting, and taxonomic classification in one step.