Shoreline Rapid Prep
$82.00 – $1,556.00
Shoreline Biome’s Rapid Prep kit, is a fast and easy-to-use kit designed for the efficient and unbiased isolation of high molecular weight (HMW) single-stranded DNA (ssDNA) in excess of 40kB from Gram-negative bacteria, Gram-positive bacteria, stool samples, and vegetive fungal cell isolates.
Shoreline Rapid Prep is a fast and easy-to-use kit designed for efficient and unbiased isolation of high molecular weight (HMW) single-stranded DNA (ssDNA). Isolate DNA in excess of 40 kB from Gram-negative bacteria, Gram-positive bacteria, stool samples, and vegetive fungal cell isolates. The ssDNA output is ideal for downstream PCR applications targeting specific bacterial or fungal genes of interest. The patented* lysis/DNA purification protocol ensures that no microbe is left behind without the need for cumbersome bead beating, while preserving DNA integrity.
*patent pending
Features:
• Ideal for stool samples and bacterial & vegetive fungal cell isolates
• Fast and easy—96 samples ready for PCR in less than 40 minutes.
• Simple automation-ready workflow.
• Superior lysis with no bead beating
• HMW ssDNA ideal for PCR applications targeting microbial or fungal genes of interest**
**Note: For downstream 16S assays targeting the V4, V1-V3, V1-V9 or 16S-23S regions, prep reagents are included in the Shoreline Complete line of kits for microbiome PCR and NGS analysis.
What is included:
• 96-well plate OR 2×8-well strip tubes with dried-down lysis reagents
• Purification buffer with magnetic beads
What you need:
• 96-well plates with plate seals OR 8-well tube strips
• TE Buffer, ETOH and KOH
• Thermocycler and magnetic plate
• Centrifuge with plate adaptor
| Weight | 1 lbs |
|---|---|
| Dimensions | 6.25 × 3.3375 × 4.25 in |
Protocols
Please complete the form below to download protocols. If you have a sample type that is not listed, please contact support@shorelinebiome.com for instructions on kit compatibility and best practices.
FAQs
Shoreline Rapid Prep
Yes, we have used the Invitrogen DynaMag™-96 Side Magnet (Cat. No. 12331D) on Lysis-1 tubes with success.
Typically for fecal samples you get 100-1000 ng of single stranded DNA, but this varies by sample type. For more information, contact support@shorelinebiome.com
You will need a magnet for handling the magnetic beads (Invitrogen DynaMag 96 side magnetic rack, #12331D or Magnetic stand-96 #AM10027). Please note that we strongly recommend the Invitrogen DynaMag 96 magnet. We’ve found that users have had the most success with this type of magnet. The Magnetic stand-96 (Thermo Fisher #AM10027) is the only other approved magnet. Plate magnets that have a ‘well’ type magnet rather than a ‘bar’ or ‘post’ will result in low DNA yield. Other required items common to most labs and are listed in each protocol.
Researchers can choose their own microbiome controls. We have several that we’ve used successfully from Zymo and ATCC.
- Process positive control: ATCC MSA-2002, MSA2006, Zymo D6300
- Process negative control: water at the lysis step
- PCR positive control: MSA 1002, MSA1006, Zymo D6305
- PCR negative control: water at the PCR step
Shoreline Biome recommends including controls in your project to monitor lysis and amplification efficiency, as well as environmental contamination.
The DNA isolated from the Rapid Prep is single stranded DNA and we recommend that this DNA be used for PCR applications only.
The Shoreline Rapid Prep kits contain:
- a 96-well plate or 16 tubes for lysis
- 2 bottles or tubes of DNA purification beads
Shoreline Biome kits have been optimized for low-sample inputs. Adding too much sample could cause the assay to fail. The following guidelines are recommended for sample input lysis:
| Sample Type | Input Amount | Maximum |
| Solid fecal | 1-3 mg | 10 mg |
| Liquid fecal | 10 µL | 10 µL |
| DNA Genotek OMNIgene®•GUT | 10 µL | 10 µL |
| Tissue | 20 mg | 30 mg |
| Mouse fecal | 5 mg (1/4 pellet) | 10 mg (1/2 pellet) |
| Bacterial Cells | 10^6 | 10^8 |
Buffer Considerations: The Shoreline Rapid Prep and Shoreline Complete lysis step is pH dependent, therefore it is important to follow recommended guidelines for sample input. If sample is stored in buffer with pH < 6.5, 10 µL is the maximum input volume. Adding more than this volume can result in suboptimal lysis and affect downstream results.
The Shoreline Biome Rapid Prep kit yields single-stranded DNA (ssDNA).
Shoreline Complete and Shoreline Rapid Prep kits have been validated on a variety of samples, as shown below. The protocol will vary depending on sample type.
| Sample Type | Protocol |
| Solid fecal Liquid fecal stored in other buffers DNA Genotek OMNIgene®•GUT Tissue Samples Mouse fecal pellet | Protocol 1 |
| Swabs Saliva Pelleted cells | Protocol 2 |
For sample types not listed, please contact support@shorelinebiome.com
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