Shoreline Complete V1-V3 Kit

Name: Shoreline Complete V1-V3 Kit
SKU: 218
Price: 1925.00 USD
Availability: InStock

$1,925.00

Simplified workflow and superb medium-resolution microbiome profiling for sequencing on Illumina platforms.

Shoreline Complete is the fastest, easiest, most complete microbiome sample prep kit on the market. The patented* lysis/DNA purification step ensures that no microbe is left behind without the need for cumbersome bead beating. The smart primer design and unique dual-end barcoding reduces read errors, virtually eliminates index hopping, and reduces amplification bias. A single-step PCR adds dual-end barcodes and Illumina adapters—just pool and sequence.

Download protocols.

SKU: N/A Categories: Illumina, Kits

Features:

Simple workflow
Superior lysis with no bead beating
HMW ssDNA ideal for PCR
Ideal for low sample inputs
One-step PCR adds barcodes and Illumina sequencing adapters
Smart PCR primer design reduces bias
Unique dual-end barcodes reduce read errors and minimize index hopping
Sample types include stool, skin, swab, tissue, soil, insect, and dairy

What is included:

Lysis plate with dried-down reagents
Purification buffer with magnetic beads
PCR plate with unique dual-end barcoded primers
2x PCR pre-mix

What you need:

96-well plates with plate seals
Thermocycler and magnetic plate
PCR purification kit
Centrifuge with plate adaptor
Gel reagents for QC

Weight	1 lbs
Dimensions	6.25 × 3.375 × 4.25 in

Barcodes

Shoreline Biome Complete kits use unique dual indexed barcodes. The barcodes are in the proper format for sequencing on the Miseq instrument and can be copied and pasted directly from the barcode file into the Illumina sample sheet. When adding the barcodes to your Illumina sample sheet, the index(i7) column from the barcodes file should be added to the “index” column of the sample sheet and the index2 (i5) column of the barcode file should be added to the “index2” column of the sample sheet. Please complete the form below to download barcodes.

Protocols

Please complete the form below to download protocols. Use Protocol 1 for preparation of fecal and tissue samples. Use Protocol 2 for preparation of low input samples including saliva, skin swabs, cell and pellets. If you have a sample type that is not listed, please contact support@shorelinebiome.com for instructions on kit compatibility and best practices.