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Shoreline Complete V1-V3 Kit
$1,925.00
Simplified workflow and superb medium-resolution microbiome profiling for sequencing on Illumina platforms.
Shoreline Complete is the fastest, easiest, most complete microbiome sample prep kit on the market. The patented* lysis/DNA purification step ensures that no microbe is left behind without the need for cumbersome bead beating. The smart primer design and unique dual-end barcoding reduces read errors, virtually eliminates index hopping, and reduces amplification bias. A single-step PCR adds dual-end barcodes and Illumina adapters—just pool and sequence.
Features:
- Simple workflow
- Superior lysis with no bead beating
- HMW ssDNA ideal for PCR
- Ideal for low sample inputs
- One-step PCR adds barcodes and Illumina sequencing adapters
- Smart PCR primer design reduces bias
- Unique dual-end barcodes reduce read errors and minimize index hopping
- Sample types include stool, skin, swab, tissue, soil, insect, and dairy
What is included:
- Lysis plate with dried-down reagents
- Purification buffer with magnetic beads
- PCR plate with unique dual-end barcoded primers
- 2x PCR pre-mix
What you need:
- 96-well plates with plate seals
- Thermocycler and magnetic plate
- PCR purification kit
- Centrifuge with plate adaptor
- Gel reagents for QC
| Weight | 1 lbs |
|---|---|
| Dimensions | 5 × 5 × 4 in |
Protocols
Please complete the form below to download protocols. Use Protocol 1 for preparation of fecal and tissue samples. Use Protocol 2 for preparation of low input samples including saliva, skin swabs, cell and pellets. If you have a sample type that is not listed, please contact support@shorelinebiome.com for instructions on kit compatibility and best practices.
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