New Version 3.0
Shoreline Biome’s unique microbiome kits are the key to generating long-read next-generation sequencing data necessary to discriminate microbial strains from any sample. The SBanalyzer software enables discovery of never before detected species and strains from microbiome samples. In addition, SBanalyzer simplifies taxonomic assignment from the Shoreline Biome full length 16S (V1-V9) or 16S-ITS-23S (StrainID™) long amplicon kits using the proprietary Athena™ database built from curated contiguous 16S-ITS-23S sequences. Shoreline Biome’s ‘Expert User Protocol’ details the exact steps needed in conjunction with DADA2 for ASV inference/database-independent identification and tracking of novel sequences or strains within and across experiments. Together, these are the Shoreline Complete™ solution.
Features
- Fast and easy to use point-and-click interface
- Easily run on a laptop or a CentOS command line
- Automated demultiplexing of V1-V9 or 16S-ITS-23S (StrainID ™) long-read output
- Accurate read mapping to proprietary Athena ™ or SILVA Datavase
- Categorization of both known and novel strains
- Simple excel based and FASTQ file data outputs for comparing microbe taxa
- Straight forward read extraction of interesting taxomnomies
- Expert User protocol with R-script enables ASV assignment using DADA2
