Kit isolates high molecular weight single-stranded DNA efficiently, without bias
FARMINGTON, Conn.— Shoreline Biome, a microbiome research company that develops tools for characterizing microbiome populations down to the strain level, recently unveiled a novel DNA isolation product.
Shoreline Rapid Prep is a fast and easy-to-use kit designed for efficient and unbiased isolation of high molecular weight (HMW) single-stranded DNA (ssDNA) in excess of 40 kB from Gram-negative bacteria, Gram-positive bacteria, and fungi in microbiome samples, cell suspensions, or cell pellets. The ssDNA output is ideal for downstream PCR applications targeting specific bacterial or fungal genes of interest.
The lysis/DNA purification protocol (patent pending) ensures that no microbe is left behind, without the need for cumbersome bead beating, while preserving DNA integrity.
The features of the kit include:
- Ideal for stool samples and bacterial and vegetative fungal cell isolates
- Fast and easy: 96 samples ready for PCR in less than 40 minutes
- Simple automation-ready workflow
- Superior lysis with no bead beating
- HMW ssDNA ideal for PCR applications targeting microbial or fungal genes of interest.
The Shoreline Complete line of kits are all-in-one kits that contain the Rapid Prep reagents and all reagents necessary for targeting the V4, V1-V3, V1-V9 or 16S-23S regions for Illumina or PacBio sequencing.
Characterizing the human microbiome and analyzing its role in human health and disease are priority goals for researchers around the world. Shoreline Biome accelerates breakthroughs in microbiome research by developing transformative discovery tools that characterize microbiome populations down to the strain level. With Shoreline Biome products, all it takes is three easy steps: lyse, purify, and amplify. Shoreline Biome’s easy-to-use companion analysis software and comprehensive reference database enables straightforward strain-level identification and quantitation of all bacteria in the sample. To learn more, visit shorelinebiome.com.
For more information contact:
Bill McKenzie, CEO